Laundry detergent compatibility of the alkaline protease from Bacillus cereus

The endogenous protease activity in various commercially available laundry detergents of international companies was studied. The maximum protease activity was found at 50degreesC in pH range 10.5-11.0 in all the tested laundry detergents. The endogenous protease activity in the tested detergents retained up to 70% on incubation at 40degreesC for 1 h, whereas less than 30% activity was only found on incubation at 50degreesC for 1 h. The alkaline protease from an alkalophilic strain of Bacillus cereus was studied for its compatibitity in commercial detergents. The cell free fermented broth from shake flask culture of the organism showed maximum activity at pH 10.5 and 50degreesC. The protease from B. cereus showed much higher residual activity (more than 80%) on incubation with laundry detergents at 50degreesC for 1 h or longer. The protease enzyme from B. cereus was found to be superior over the endogenous proteases present in the tested commercial laundry detergents in comparison to the enzyme stability during the washing at higher temperature, e.g., 40-50degreesC. (C) 2004 Elsevier GmbH. All rights reserved.