Journal
NEURON GLIA BIOLOGY
Volume 1, Issue -, Pages 113-126Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S1740925X04000213
Keywords
Stem cells; neurons; glial cells; neural progenitors; spinal cord injury
Categories
Funding
- IRTA
- NIH [NS24707, NS 37515]
- NIA
- CNS Foundation
- Packard Center
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [P50NS024707, R01NS037515] Funding Source: NIH RePORTER
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Multiple classes of precursor cells have been isolated and characterized from the developing spinal cord including multipotent neuroepithelial (NEP) stern cells and lineage-restricted precursors for neurons (NRPs) and glia (GRPs). We have compared the survival, differentiation and integration of multipotent NEP cells with lineage-restricted NRPs and GRPs using cells isolated from transgenic rats that express the human placental alkaline phosphatase gene. Our results demonstrate that grafted NEP cells survive poorly, with no cells observed 3 days after transplant in the adult hippocampus, striatum and spinal cord, indicating that most CNS regions are not compatible with transplants of multipotent cells derived from fetal CNS. By contrast, at 3 weeks and 5 weeks post-engraftment, lineage-restricted precursors showed selective migration along white-matter tracts and robust survival in all three CNS regions. The grafted precursors expressed the mature neuronal markets NeuN and MAP2, the astrocytic marker GFAP, the oligodendrocytic markets RIP, NG2 and Sox-10, and the synaptic marker synaptophysin. Similar behavior was observed when these precursors were transplanted into the injured spinal cord. Predifferentiated, multipotent NEP cells also survive and integrate, which indicates that lineage-restricted CNS precursors are well suited for transplantation into the adult CNS and provide a promising cellular replacement candidate.
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