Journal
NEURON GLIA BIOLOGY
Volume 1, Issue -, Pages 3-11Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S1740925X04000031
Keywords
Fluo-4; GFAP; photolysis; caged glutamate; glia; calcium
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Funding
- National Institutes of Health [RO1 NS43142, R37 NS37585]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM072910] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R01NS043142, R01NS037585, R37NS037585] Funding Source: NIH RePORTER
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Little is known about the functional connectivity between astrocytes in the CNS. To explore this issue we photo-released glutamate onto a single astrocyte in murine hippocampal slices and imaged calcium responses. Photo-release of glutamate causes a metabotropic glutamate receptor (mGluR)-dependent increase in internal calcium in the stimulated astrocyte and delayed calcium elevations in neighboring cells. The delayed elevation in calcium was not caused by either neuronal activity following synaptic transmission or by glutamate released from astrocytes. However, it was reduced by flufenamic acid (FFA), which is consistent with a role for adenosine triphosphate (ATP) release from astrocytes as an intercellular messenger. Exogenous ligands such as ATP (1 mu M) increased the number of astrocytes that were recruited into coupled astrocytic networks, indicating that extracellular accumulation of neurotransmitters modulates neuronal excitability, synaptic transmission and functional coupling between astrocytes.
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