Journal
MOLECULAR BIOLOGY OF THE CELL
Volume 15, Issue 1, Pages 71-80Publisher
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E03-08-0586
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Funding
- NCI NIH HHS [CA77325, R01 CA077325, CA81665] Funding Source: Medline
- NCRR NIH HHS [RR11823, P41 RR011823] Funding Source: Medline
- NEI NIH HHS [R01 EY1328801] Funding Source: Medline
- NIGMS NIH HHS [GM59477] Funding Source: Medline
- NATIONAL CANCER INSTITUTE [R01CA077325, R33CA081665] Funding Source: NIH RePORTER
- NATIONAL CENTER FOR RESEARCH RESOURCES [P41RR011823] Funding Source: NIH RePORTER
- NATIONAL EYE INSTITUTE [R01EY013288] Funding Source: NIH RePORTER
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In most eukaryotes, genes encoding ribosomal RNAs (rDNA) are clustered in long tandem head-to-tail repeats. Studies of Saccharomyces cerevisiae have indicated that rDNA copy number is maintained through recombination events associated with site-specific blockage of replication forks (RFs). Here, we describe two Schizosaccharomyces pombe proteins, homologs of S. cerevisiae Slx1 and Slx4, as subunits of a novel type of endonuclease that maintains rDNA copy number. The Slx1-Slx4-dependent endonuclease introduces single-strand cuts in duplex DNA on the 3' side of junctions with single-strand DNA. Deletion of Slx1 or Rqh1 RecQ-like DNA helicase provokes rDNA contraction, whereas simultaneous elimination of Slx1-Slx4 endonuclease and Rqh1 is lethal. Slx1 associates with chromatin at two foci characteristic of the two rDNA repeat loci in S. pombe. We propose a model in which the Slx1-Slx4 complex is involved in the control of the expansion and contraction of the rDNA loci by initiating recombination events at stalled RFs.
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