Synthesis and structure of 5,5-diethoxycarbonyl-l-pyrroline N-oxide (DECPO). Application to superoxide radical trapping

DECPO, a new analogue of EMPO was synthesized through a two-step synthetic pathway. Its structure and its application to trap superoxide were investigated. The ESR detection of the DECPO-OOH spin adduct is easy even at low concentration Of Superoxide. In comparison with DEPMPO, the trapping of superoxide with DECPO is faster and the detection of DECPO-OOH can be performed using a very low nitrone concentration (0.5 mM). (C) 2003 Elsevier Ltd. All rights reserved.