Journal
JOURNAL OF VIROLOGY
Volume 78, Issue 2, Pages 642-649Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.78.2.642-649.2004
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Funding
- NATIONAL CANCER INSTITUTE [P30CA007175, P01CA022443, P30CA014520] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R01AI060018] Funding Source: NIH RePORTER
- NCI NIH HHS [P01 CA22443, P30 CA07175, P01 CA022443, P30 CA14520, P30 CA014520] Funding Source: Medline
- NIAID NIH HHS [R01 AI060018] Funding Source: Medline
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Synthesis of the relaxed-circular (RC) genome of hepadnaviruses is a multistep process that requires template switching during reverse transcription. Studies of duck hepatitis B virus indicated the presence of cis-acting sequences, distinct from the donor and acceptor sequences for the template switches, which contribute to the synthesis of RC DNA. However, knowledge about cis-acting requirements distinct from the donor and acceptor sites for human hepatitis B virus (HBV) was lacking. In this study, we searched for cis-acting sequences for synthesis of HBV RC DNA by analyzing a set of deletion variants that collectively represent most of the HBV genome. Sequences of epsilon, DR1, DR2, 5'r, and 3'r were not analyzed in the study. Results from Southern blotting showed that multiple cis-acting sequences were involved in the synthesis of HBV RC DNA. Analysis of several HBV/woodchuck hepatitis virus chimeras corroborated the findings from the analysis of deletion variants. This study represents a comprehensive and quantitative analysis of cis-acting sequences that contribute to the synthesis of HBV RC DNA.
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