Journal
PLANT AND CELL PHYSIOLOGY
Volume 45, Issue 5, Pages 608-617Publisher
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pch069
Keywords
Ca2+; CDPK; dephosphorylation; petal oscillation; phosphorylation; water transport
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The opening and closing of tulip petals was reproduced in the dark by changing the temperature from 5 degreesC to 20 degreesC for opening and 20 degreesC to 5 degreesC for closing. The opening process was accompanied by (H2O)-H-3 transport through the stem from the incubation medium to the petals. A Ca(2+)channel blocker and a Ca2+-chelator inhibited petal opening and (H2O)-H-3 transport. Several proteins in the isolated plasma membrane fraction were phosphorylated in the presence of 25 muM Ca2+ at 20 degreesC. The 31-kDa protein that was phosphorylated, was suggested immunologically as the putative plasma membrane aquaporin (PM-AQP). This phosphorylated PM-AQP clearly reacted with the anti-phospho-Ser. In-gel assay revealed the presence of a 45-kDa Ca2+-dependent protein kinase in the isolated plasma membrane. Phosphorylation of the putative PM-AQP was thought to activate the water channel composed of PM-AQP Dephosphorylation of the phosphorylated PM-AQP was also observed during petal closing at 5 degreesC, suggesting the inactivation of the water channel.
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