Journal
JOURNAL OF NEUROIMMUNOLOGY
Volume 146, Issue 1-2, Pages 13-21Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jneuroim.2003.09.016
Keywords
morphine; lipopolysaccharide; vascular endothelial cells; permeability; apoptosis; Fas
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Funding
- NATIONAL INSTITUTE ON DRUG ABUSE [R01DA007058, K02DA016149] Funding Source: NIH RePORTER
- NIDA NIH HHS [R01 DA 07058, KO2 DA 016149] Funding Source: Medline
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Vascular endothelial cells (VEC) provide an essential protective barrier between the vascular system and underlying tissues. Using VEC barrier models of human coronary artery cells and human and rat brain microvascular endothelial cells, we investigated the mechanism by which morphine affects lipopolysaccharide (LPS)-induced VEC permeability. We demonstrated that co-administration of morphine and LPS induced greater VEC apoptosis and permeability than morphine or LPS alone. The extent of induced apoptosis appeared to be cell-type dependent. Furthermore, RT-PCR analysis revealed that morphine and LPS up-regulated Fas expression. These data suggest potential crosstalk between the signaling pathways that mediate morphine- and LPS-triggered apoptosis in brain VEC. (C) 2003 Published by Elsevier B.V.
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