Journal
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
Volume 18, Issue 4, Pages 491-498Publisher
JOHN WILEY & SONS LTD
DOI: 10.1002/rcm.1364
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Direct quantitation of glutathione S-transferase (GST) isoforms [alpha (GST-A) and mu (GST-M)]in human liver cytosol was achieved by liquid chromatography/tandem mass spectrometry (LC/ESI-MS/MS) analysis of signature peptides of GST-A and GST-M and their corresponding stable isotopic peptide internal standards via multiple reaction monitoring (MRM). The selection of signature peptides was performed via trypsin digestion of commercially available cDNA-expressed GST-A1 and GST-M1, followed by LC/ESI-MS/MS with an ion trap mass spectrometer and sequencing with the TurboSEQUEST((R)) application. Quantitative analysis of the selected signature peptides in the multi-reaction monitoring (MRM) mode was performed using a triple-quadruple mass spectrometer. A series of human cytosol samples was quantitatively analyzed for levels of GST-A and GST-M. The total level of GST-A and GST-M obtained from this LC/ESI-MS/MS method was well correlated with the total level of GST determined by the 1-chloro-2,4-dinitrobenzene (CDNB) method. Copyright (C) 2004 John Wiley Sons, Ltd.
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