Journal
CARDIOVASCULAR RESEARCH
Volume 83, Issue 3, Pages 547-557Publisher
OXFORD UNIV PRESS
DOI: 10.1093/cvr/cvp153
Keywords
TRPM7; Vascular endothelial cells; MAPK; Growth; proliferation
Categories
Funding
- National Institutes of Health [R01NS42926, R01NS49470]
- American Heart Association Established Investigator Award [0840132]
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The presence and potential function of transient receptor potential melastatin 7 (TRPM7), a Ca(2+)-permeable non-selective cation channel of the TRP channel superfamily in human vascular endothelial cells, were examined. Whole-cell patch-clamp recordings showed outward-rectifying currents in human umbilical vein endothelial cells (HUVECs), which was potentiated by removing the extracellular Ca(2+) and Mg(2+), but inhibited by non-specific TRPM7 blocker Gd(3+) or 2-aminoethoxydiphenyl borate (2-APB). TRPM7 mRNA was detected in HUVECs by RT-PCR, but TRPM6, its closest homologue, was not. Silencing TRPM7 by small interfering RNA (siRNA) decreased the level of TRPM7 mRNA and the TRPM7-like current. Interestingly, knockdown of TRPM7 with siRNA or inhibition of TRPM7 function with 2-APB increased the phosphorylation of extracellular signal-regulated kinase (ERK) and enhanced growth/proliferation of HUVECs. This enhanced cell growth/proliferation was abolished by an inhibitor of the ERK signalling pathway. In addition to cell growth/proliferation, silencing TRPM7 also increased expression of nitric oxide synthase and nitric oxide production in an ERK pathway-dependent manner. These observations suggest that TRPM7 channels may play an important role in the function of vascular endothelial cells.
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