Journal
CARDIOVASCULAR RESEARCH
Volume 83, Issue 1, Pages 140-147Publisher
OXFORD UNIV PRESS
DOI: 10.1093/cvr/cvp108
Keywords
CRP3/MLP; Arterialized vein graft; Myocardial revascularization; Saphenous vein; Stretch
Categories
Funding
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo-FAPESP [01/00009-0, 03/14115-2, 00/09485-7, 06/52053-7]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico CNPq [478073/2004-6]
Ask authors/readers for more resources
Aims Cysteine- and glycine-rich protein 3/muscle LIM-domain protein (CRP3/MLP) mediates protein-protein interaction with actin filaments in the heart and is involved in muscle differentiation and vascular remodelling. Here, we assessed the induction of CRP3/MLP expression during arterialization in human and rat veins. Methods and results Vascular CRP3/MLP expression was mainly observed in arterial samples from both human and rat. Using quantitative real time RT-PCR, we demonstrated that the CRP3/MLP expression was 10 times higher in smooth muscle cells (SMCs) from human mammary artery (h-MA) vs. saphenous vein (h-SV). In endothelial cells (ECs), CRP3/MLP was scarcely detected in either h-MA or h-SV. Using an ex vivo flow through system that mimics arterial condition, we observed induction of CRP3/MLP expression in arterialized h-SV. Interestingly, the upregulation of CRP3/MLP was primarily dependent on stretch stimulus in SMCs, rather than shear stress in ECs. Finally, using a rat vein in vivo arterialization model, early (1-14 days) CRP3/MLP immunostaining was observed predominantly in the inner layer and later (28-90 days) it appeared more scattered in the vessel layers. Conclusion Here we provide evidence that CRP3/MLP is primarily expressed in arterial SMCs and that stretch is the main stimulus for CRP3/MLP induction in veins exposed to arterial haemodynamic conditions.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available