Journal
PROTEOMICS
Volume 4, Issue 1, Pages 257-260Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/pmic.200300546
Keywords
dog; gammopathy; human; immunoglobulin; nonreducing two-dimensional gel electrophoresis
Ask authors/readers for more resources
Nonreducing two-dimensional gel electrophoresis (2-DE) is described for the study of immunoglobulin disorders with asynchronous production of single chains. Unlike classical reducing 2-DE, this method can distinguish between complex intact molecules and their free single chains (with different degrees of polymerization) and will thus be helpful for diagnosis of this type of disease. Examples are taken from canine patients, but the method may also be applied to both urine and serum specimens from other species. Nonreducing 2-DE thus represents a useful tool complementary to classical 2-DE, when further information about the appearance of free subunits or modifications of proteins are required, even in the presence of intact molecules.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available