Journal
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
Volume 42, Issue 12, Pages 1377-1383Publisher
WALTER DE GRUYTER GMBH
DOI: 10.1515/CCLM.2004.257
Keywords
dimethylarginine; endothelial dysfunction; inhibitor; NO pathway; risk factor
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Funding
- NCCIH NIH HHS [R01 AT/HL00204] Funding Source: Medline
- NHLBI NIH HHS [R01 HL-75774, R01 HL-63685] Funding Source: Medline
- NIAID NIH HHS [P011AI50153] Funding Source: Medline
- NIA NIH HHS [P01 AG18784] Funding Source: Medline
- NATIONAL CENTER FOR COMPLEMENTARY &ALTERNATIVE MEDICINE [R01AT000204] Funding Source: NIH RePORTER
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL075774, R01HL063685] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [P01AI050153] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON AGING [P01AG018784] Funding Source: NIH RePORTER
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Asymmetric dimethylarginine (ADMA) is an endogenous competitive inhibitor of nitric oxide synthase (NOS). Elevated ADMA plasma levels have been reported in connection with diseases associated with an impaired endothelial L-arginine-NO pathway and endothelial dysfunction, such as atherosclerosis, hypercholesterolemia, chronic heart failure, diabetes mellitus, and hypertension. NO production by NOS is decreased due to elevated ADMA levels. In fact, there is increasing interest in determination of ADMA levels in samples of various origins. The aim of this work was to develop a precise and easy immunoassay in contrast to the existing methods, such as HPLC, liquid chromatography-mass spectrometry (LC-MS) and gas chromatography (GC)-MS. We determined cross-reactivity in our immunoassay of 1.2% for symmetric dimethylarginine and <0.02% for L-arginine. The limit of quantitation was 0.05 mu mol/l. We found good correlation of the values measured when we compared our assay with LC-tandem MS (n=29; r=0.984; p<0.0001). We determined ADMA levels in human serum and plasma, mouse and rat plasma, and cell culture supernatant. For human plasma we found a mean of 0.65 mumol/l in healthy subjects. In the plasma of mice and rats we found mean concentrations of 1.05 and 1.09 mumol/l, respectively.
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