Glycosaminoglycans enhance the trifluoroethanol-induced extensionof beta(2)-microglobulin-related amyloid fibrils at a neutral pH

beta(2)-Microglobulin-related (Abeta2M) amyloidosis is a frequent and serious complication in patients on long-term dialysis, and beta(2)-microglobulin is a major structural component of Abeta2M amyloid fibrils. Several biologic molecules inhibiting the depolymerization of Abeta2M amyloid fibrils at a neutral pH were found recently. The effect of trifluoroethanol and glycosaminoglycans (GAG) on the extension of the fibrils at a neutral pH was investigated with the use of fluorescence spectroscopy with thioflavin T, circular dichroism spectroscopy, and electron microscopy. Trifluoroethanol at concentrations of up to 20% (vol/vol) caused fibril extension of heparin-stabilized seeds, inducing a subtle change in the tertiary structure of beta(2)-microglobulin and stabilizing the fibrils at a neutral pH. This extension reaction followed a first-order kinetic model. In addition, some GAG, especially heparin, dose-dependently enhanced the fibril extension. These results suggest that some GAG, especially heparin, may bind to the fibrils and enhance their deposition in vivo. Thus, the experimental system described here should be useful to search for the factors that accelerate Abeta2M amyloid deposition in vivo. In addition, the interference of the binding of GAG to Abeta2M amyloid fibrils may be an attractive therapeutic modality.