Journal
CHROMATOGRAPHIA
Volume 60, Issue -, Pages S235-S238Publisher
SPRINGER HEIDELBERG
DOI: 10.1365/s10337-004-0188-x
Keywords
column liquid chromatography; Lobelia inflato; hairy root; lobetyolin; lobetyolinin
Ask authors/readers for more resources
Lobelia inflato L. contains pharmacologically important secondary metabolites. The main alkaloid is lobeline which has a stimulatory effect on the respiratory centre. Recently high quantity of polyacetylenes was determined in the plant and hairy root cultures. With the aim of increasing of the secondary metabolite production hairy root cultures were produced (by direct infection of the sterile plants with Agrobacterium rhizogenes strain R1601). A new HPLC method was developed for the determination of lobetyolin and lobetyolinin, the main polyacetylene compounds of L. inflata hairy roots. The analysis was performed on Hypersil MOS column (250 x 4 mm I.D.). The mobile phase was 15:85 (v/v) mixture of acetonitrile and water. Peaks were identified by addition of standards and by DAD. The external standardized method allowed the determination of lobetyolin and lobetyolinin with good sensitivity and reliability. This method was used to measure the polyacetylene contents of hairy roots grew intensively in bioreactor. High contents of lobetyolin (3.6%) and lobetyolinin (0.8-1.6%) were detected in the hairy roots because of their great biosynthetic capacity.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available