Journal
PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR
Volume 77, Issue 1, Pages 175-181Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.pbb.2003.10.007
Keywords
cytochrome oxidase; methylene blue; mitochondria; oxygen consumption; holeboard maze; memory retention; spatial memory; cerebral metabolism; neurodegenerative diseases
Funding
- NATIONAL INSTITUTE OF MENTAL HEALTH [T32MH018837] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R01NS037755] Funding Source: NIH RePORTER
- NIMH NIH HHS [T32 MH18837] Funding Source: Medline
- NINDS NIH HHS [R01NS37755] Funding Source: Medline
Ask authors/readers for more resources
Methylene blue (MB) increases mitochondrial oxygen consumption and restores memory retention in rats metabolically impaired by inhibition of cytochrome c oxidase. This study tested two related hypotheses using biochemical and behavioral techniques: (1) that low-level NIB would enhance brain cytochrome c oxidation, as tested in vitro in brain homogenates and after in vivo administration to rats and (2) that corresponding low-dose MB would enhance spatial memory retention in normal rats, as tested 24 It after rats were trained in a baited holeboard maze for 5 days with daily MB posttraining injections. The biochemical in vitro studies showed an increased rate of brain cytochrome c oxidation with the low but not the high MB concentrations tested. The in vivo administration studies showed that the corresponding MB low dose (1 mg/kg) increased brain cytochrome c oxidation 24 h after intraperitoneal injection, but not after I or 2 h postinjection. In the behavioral studies, spatial memory retention in probe trials (percentage of visits to training-baited holes compared to total visits) was significantly better for MB-treated than saline control groups (66% vs. 3 1 %). Together the findings suggest that low-dose MB enhances spatial memory retention in normal rats by increasing brain cytochrome c oxidase activity. (C) 2003 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available