Journal
JOURNAL OF APPLIED MICROBIOLOGY
Volume 96, Issue 5, Pages 922-930Publisher
WILEY
DOI: 10.1111/j.1365-2672.2004.02243.x
Keywords
Escherichia coli; microcosm; persistence; recreational water; sediment; survival
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Aims: To determine the persistence of the faecal indicator organism Escherichia colt in recreational coastal water and sediment using laboratory-based microcosms and validation with in situ measurements. Methods and Results: Intact sediment cores were taken from three distinct coastal sites. Overlying estuarine water was inoculated with known concentrations of E. coli and decay rates from both overlying water and sediment were determined following enumeration by the membrane filtration method at fixed time intervals over a 28-day period. It was demonstrated that E. coli may persist in coastal sediment for >28 days when incubated at 10degreesC. Escherichia colt survival was found to have an inverse relationship with temperature in both water and sediment. In general the decay rate for E. coli was greater in water than in sediment. Small particle size and high organic carbon content were found to enhance E. coli survival in coastal sediments; in the microcosms. Conclusions: Results of this microcosm study demonstrated the more prolonged survival of E. colt In coastal sediments compared with overlying water, which may imply an increased risk of exposure because of the possible resuspension of pathogenic micro-organisms during natural turbulence or human recreational activity. Significance and Impact of the Study: A more accurate estimate of exposure risk has been described which may subsequently be used in a quantitative microbial risk assessment for recreational coastal waters.
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