Journal
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
Volume 286, Issue 1, Pages L210-L220Publisher
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajplung.00332.2003
Keywords
surfactant secretion; alveolar type II cells; mechanical strain; stretch
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Using a new equibiaxial strain device, we investigated strain-induced Ca(2+) signals and their relation to lamellar body (LB) exocytosis in single rat alveolar type II (AT II) cells. The strain device allows observation of single cells while inducing strain to the entire substratum. AT II cells tolerated high strain amplitudes up to 45% increase in cell surface area (DeltaCSA) without release of lactate dehydrogenase or ATP. Strain exceeding a threshold of similar to 8% DeltaCSA resulted in a transient rise of the cytoplasmic Ca(2+) concentration in some cells. Higher strain levels increased the fraction of Ca(2+)-responding cells. The occurrence of strain-induced Ca(2+) signals depended on cell-cell contacts, because lone cells (i.e., cells without cell-cell contacts) did not exhibit Ca(2+) signals. Above threshold, the amplitude of the Ca(2+) signal as well as the number of stimulated LB fusions correlated well with the amplitude of strain. Furthermore, stimulated LB fusions occurred only in cells exhibiting a Ca(2+) signal; 50 muM Gd(3+) in the bath affected neither Ca(2+) signals nor fusions. Intracellular Ca(2+) release was triggered at higher strain amplitudes and inhibited by thapsigargin. Removal of bath Ca(2+) completely inhibited Ca(2+) signals and fusions. We conclude that strain of AT II cells stimulates a Ca(2+) entry pathway that is highly sensitive to strain and a prerequisite for subsequent Ca(2+) release. Both mechanisms result in a graded response of fusions to strain. Our data also allow us to introduce the term effective strain as the physiologically relevant portion of the strain amplitude.
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