Journal
BIOTECHNOLOGY LETTERS
Volume 26, Issue 2, Pages 153-157Publisher
SPRINGER
DOI: 10.1023/B:BILE.0000012895.60773.ff
Keywords
cysteine biosynthesis; heavy metal tolerance; phytochelatin; phytoremediation; transgenic plants
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Cysteine synthase [O-acetyl-L-serine(thiol) lyase] catalyzes the final step for L-cysteine biosynthesis in plants. The tolerance of transgenic tobacco plants over-expressing cysteine synthase cDNA in cytosol (3F), chloroplasts (4F) and in both organelles (F-1) was investigated towards heavy metals such as Cd, Se, Ni, Pb and Cu. The transgenic plants were significantly more tolerant than wild-type plants in agar medium containing Cd, Se and Ni. The F-1 transgenic plants had a higher resistance than other transgenic lines towards these metals and could enhance accumulation of Cd in shoot. These results suggest that the transgenic plants over-expressing cysteine synthase both in cytosol and chloroplasts can be applicable to phyto-remediation of Cd from contaminated soils.
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