Disposable carbon nanotube modified screen-printed biosensor for amperometric detection of organophosphorus pesticides and nerve agents

A disposable carbon nanotube-based biosensor was successfully developed and applied to the detection of organophosphorus (OP) pesticides and nerve agents. The biosensors using acetycholinesterase (AChE)/choline oxidase (CHO) enzymes provided a high sensitivity, large linear range, and low detection limits for the analysis of OP compounds. Such characteristics may be attributed to the catalytic activity of carbon nanotube to promote the redox reaction of hydrogen peroxide produced during AChE/CHO enzymatic reactions with their substrate, as well as the large surface area of carbon nanotube materials.