Journal
CARDIOVASCULAR DRUGS AND THERAPY
Volume 23, Issue 6, Pages 439-448Publisher
SPRINGER
DOI: 10.1007/s10557-009-6206-3
Keywords
Lycopene; Atherosclerosis; Oxidative stress; Endothelial cells; Apoptosis
Funding
- national foundation for natural sciences of China [30200103]
Ask authors/readers for more resources
Oxidative stress is considered to be a major factor contributing to damage of endothelial cells, and is an important component of the etiology of atherosclerosis. In this study, we investigated the effects of lycopene on the oxidative injury and apoptosis of endothelial cells induced by H2O2, and the effects of lycopene on the expression of p53, caspase-3 mRNA in injured cells. In the H2O2 group, endothelial cells were incubated with 400 A mu M H2O2. In lycopene groups, endothelial cells were pretreated with different concentrations of lycopene then exposed to 400 A mu M H2O2. In the drug control group, cells were pretreated with probucol then incubated with H2O2. The effects of different concentrations of lycopen on the extent of oxidative injury to the cells were evaluated. The growth conditions and morphological changes of the cells were observed with an inverted microscope. The level of oxidative injury to cells was determined by measuring malondialdehyde (MDA) levels; the viability of cells was detected by MTT assays; the nuclear morphology of cells was observed by Hoechst staining; the apoptotic ratio of cells was measured by flow cytometry; and the expressions of p53 and caspase-3 mRNA were investigated by RT-PCR. Lycopene improved the shape of contracted endothelial cells induced by H2O2 injury, and diminished the level of MDA produced following oxidative injury of cells. The viability of cells increased, and the number of cells characterized by apoptotic nuclear morphology decreased in groups treated with lycopene. Similarly, lycopene significantly diminished the apoptosis ratio of oxidative injured cells, and also downregulated the expressions of p53 and caspase-3 mRNA induced by H2O2. Lycopene and probucol displayed similar protective effects on endothelial cells. Lycopene can decrease the oxidative injury of endothelial cells induced by H2O2, can attenuate the expression of p53 and caspase-3 mRNA in injured cells, and can diminish the apoptosis of injured cells. These findings possibly explain in part why lycopene can prevent atherosclerotic cardiovascular diseases.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available