Journal
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
Volume 133, Issue 1, Pages 15-26Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molbiopara.2003.08.009
Keywords
malaria; Plasmodium yoelii; multigene family; gene expression; stage-specific expression; pore-forming protein; host-cell invasion
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Funding
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R01AI041879, R01AI028398] Funding Source: NIH RePORTER
- NIAID NIH HHS [R01 AI028398, AI 053709, R01 AI41879] Funding Source: Medline
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Pore-forming proteins are employed by many pathogens to achieve successful host colonization. Intracellular pathogens use pore-forming proteins to invade host cells, survive within and productively interact with host cells, and finally egress from host cells to infect new ones. The malaria-causing parasites of the genus Plasmodium evolved a number of life cycle stages that enter and replicate in distinct cell types within the mosquito vector and vertebrate host. Despite the fact that interaction with host-cell membranes is a central theme in the Plasmodium life cycle, little is known about parasite proteins that mediate such interactions. We identified a family of five related genes in the genome of the rodent malaria parasite Plasmodium yoelii encoding secreted proteins all bearing a single membrane-attack complex/perforin (MACPF)-like domain. Each protein is highly conserved among Plasmodium species. Gene expression analysis in P yoelli and the human malaria parasite Plasmodium falciparum indicated that the family is not expressed in the parasites blood stages. However, one of the genes was significantly expressed in P. yoelli sporozoites, the stage transmitted by mosquito bite. The protein localized to the micronemes of sporozoites, organelles of the secretory invasion apparatus intimately involved in host-cell infection. MACPF-like proteins may play important roles in parasite interactions with the mosquito vector and transmission to the vertebrate host. (C) 2003 Elsevier B.V. All rights reserved.
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