Journal
JOURNAL OF MAGNETIC RESONANCE
Volume 166, Issue 1, Pages 129-133Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jmr.2003.09.004
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Here we report a modified pulsed gradient spin-echo (PGSTE) pulse sequence to measure diffusion coefficients. This approach incorporates WATERGATE combined with isotopic filtering into a standard PGSTE experiment. Doing this eliminates much of the disadvantages from the combination of diffusion encoding and heteronuclear selection intervals and allows for facile modification of the diffusion pulse sequence with flexibility of the time period between RF pulses. The new diffusion pulse sequence is demonstrated using an N-15-labeled peptide and an N-15-labeled protein in a mixture with a protein of similar size. (C) 2003 Elsevier Inc. All rights reserved.
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