Journal
JOURNAL OF BIOMOLECULAR NMR
Volume 28, Issue 1, Pages 59-67Publisher
SPRINGER
DOI: 10.1023/B:JNMR.0000012861.95939.05
Keywords
hTR; isotope filter; NMR; NOESY; RNA; structure; telomerase
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Funding
- NATIONAL CANCER INSTITUTE [F32CA091643] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM037254] Funding Source: NIH RePORTER
- NCI NIH HHS [CA 91643] Funding Source: Medline
- NIGMS NIH HHS [GM 37254] Funding Source: Medline
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NMR spectra of large RNAs are difficult to assign because of extensive spectral overlap and unfavorable relaxation properties. Here we present a new approach to facilitate assignment of RNA spectra using a suite of four 2D-filtered/ edited NOESY experiments in combination with base-type-specific isotopically labeled RNA. The filtering method was developed for use in 3D filtered NOESY experiments ( Zwahlen et al., 1997), but the 2D versions are both more sensitive and easier to interpret for larger RNAs than their 3D counterparts. These experiments are also useful for identifying intermolecular NOEs in RNA-protein complexes. Applications to NOE assignment of larger RNAs and an RNA-protein complex are presented.
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