Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 284, Issue 1-2, Pages 141-145Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2003.09.018
Keywords
oligoclonal IgG bands; multiple sclerosis; isoelectrofocusing; western blotting
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We have developed an ultrasensitive isoelectrofocusing (IEF) method for the detection of oligoclonal (OGC) IgG bands in cerebrospinal fluid (CSF) and sera. In this procedure, double antibody and peroxidase immunodetection have been substituted by a single antibody labelled with alkaline phosphatase. Alkaline phosphatase immunodetection not only improves tenfold the sensitivity of the assay but also gives a sharper pattern resolution making the interpretation easier and increases the specificity of this technique. This preliminary report should be validated in a larger number of patients with and without multiple sclerosis (MS). (C) 2003 Elsevier B.V. All rights reserved.
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