Protective and immunomodulatory effect of flos Lonicerae japonicae by augmenting IL-10 expression in a murine model of acute lung inflammation

Ethnopharmacological relevance: Lonicerae japonicae has shown antibacterial, anti-inflammatory, antipyretic, antioxidant, antiviral, and protective effects in animal models of chronic obstructive pulmonary disease. Aim of the study: To investigate the effects of Flos L japonicae (FLJ), a traditional Chinese medicinal herb, on acute lung inflammation induced by lipopolysaccharide (LPS), in vivo, using a murine model. Materials and methods: Thirty 6-week-old female BALB/c mice were challenged with intratracheal LPS before treatment with FLJ or vehicle. FLJ was examined for its capacity to influence an LPS-induced increase in IL-10 and decrease in TNF-alpha, IL-1 beta and IL-6 as measured in murine bronchoalveolar lavage (BALF). Results: FLJ increased nuclear Spl binding activity thereby enhancing the expression of IL-10 and decreased nuclear NF-kappa B binding activities thereby inhibiting the expression of TNF-alpha, IL-1 beta and IL-6 in the lung. The up-regulation of Spl activity by FLJ was through incremental phosphorylation of ERK. By contrast, the down-regulation of NF-kappa B activity by FLJ was through suppression of the phosphorylation of 1 kappa B, p38, and JNK. Conclusions: We demonstrated FLJ has protective activity against LPS-induced lung inflammatory cytokine release. Anti-inflammatory cytokine, IL-10, may prove beneficial in the treatment of endotoxin-associated lung inflammation. (C) 2015 Elsevier Ireland Ltd. All rights reserved.