Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 296, Issue 1-2, Pages 31-36Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2004.10.008
Keywords
electroporation; NK cells; transient expression; RNAi
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Funding
- NATIONAL CANCER INSTITUTE [P01CA078582] Funding Source: NIH RePORTER
- NATIONAL CENTER FOR RESEARCH RESOURCES [P20RR016434] Funding Source: NIH RePORTER
- NCI NIH HHS [P01 CA78582] Funding Source: Medline
- NCRR NIH HHS [P20 RR16434] Funding Source: Medline
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Introducing genes into cells is a crucial step in many fields of basic research, as well as for the development of new drugs and therapies, Many cell types are resistant to normal methods of gene delivery, such as lipid based transfection and electroporation. Delivery to resistant cell lines can be costly or inefficient. Natural killer (NK) cells are highly resistant to transfection. We have developed a novel method to deliver exogenous genes in the NK cell line, NK92. Using a combination of electroporation and a defined buffer, we were able to obtain an electroporation efficiency of 40% in NK92 cells. Using RNAi, we show significant reduction of an endogenous protein (ETS1) using this optimized buffer and electroporation conditions. Taken to.-ether, the results show a functional and cost effective method for the expression of exogenous genes in NK cells. (C) 2004 Elsevier B.V. All rights reserved.
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