Journal
JOURNAL OF BIOCHEMISTRY
Volume 137, Issue 1, Pages 33-39Publisher
JAPANESE BIOCHEMICAL SOC
DOI: 10.1093/jb/mvi007
Keywords
catalytic domain; expression cloning; immunoscreening; Lotus japonicus; protein kinase
Categories
Ask authors/readers for more resources
To investigate protein kinases expressed in Lotus japonicus, a cDNA expression library of the root-nodule of L. japonicus was immunologically screened with monoclonal antibodies directed to a highly conserved region in protein serine/threonine kinases (Ser/Thr kinases). Among 178 positive clones obtained from the lambda ZAPII cDNA library, 164 clones were found to encode novel proteins possessing the subdomain VIE sequences characteristic of Ser/Thr kinases. By phylogenetic analysis on the basis of deduced amino acid sequences, the isolated clones could be classified into five different families of Ser/Thr kinases : the SnRK family, GSK-3 family, Ndr kinase family, Ark family, and receptor kinase family. These results suggest that this expression cloning using the kinase-specific antibodies will provide new clues for investigations of a wide variety of known and novel protein kinases in higher plants.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available