Journal
LAB ON A CHIP
Volume 5, Issue 11, Pages 1327-1330Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b509086e
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A bead-based microfluidic device was developed and demonstrated to achieve rapid and sensitive enzyme-linked immunosorbent assay ( ELISA) with quantum dots as the labeling fluorophore for virus detection. In comparison to standard ELISA performed on the same virus, the minimal detectable concentration of the target virus was improved from 360 to 22 ng mL(-1), the detection time was shortened from >3.25 h to <30 min, and the amount of antibody consumed was reduced by a factor of 14.3.
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