Journal
EUPHYTICA
Volume 142, Issue 1-2, Pages 161-167Publisher
SPRINGER
DOI: 10.1007/s10681-005-1251-x
Keywords
breakpoint interval (bin) mapping; genetic mapping; molecular markers; powdery mildew; resistance genes; wheat
Categories
Ask authors/readers for more resources
The Triticum dicoccoides-derived wheat line Zecoi-1 provides effective protection against powdery mildew. F-3 segregation analysis of Chinese Spring x Zecoi-1 hybrids showed that resistance in line Zecoi-1 is controlled by a single dominant gene. Amplified fragment length polymorphism (AFLP) analysis of bulked segregants from F(3)s showing the homozygous resistant and susceptible phenotypes identified eight markers, of which four were associated with the resistance allele in repulsion phase. Following the assignment of these four repulsion phase AFLP markers to wheat chromosome 2B with the aid of Chinese Spring nulli-tetrasomic lines, they were physically mapped in the terminal breakpoint interval 0.89 (2BL-6)-1.00 (telomere) of chromosome 2BL. Genetic and physical mapping of simple sequence repeat markers from the distal half of chromosome 2BL located the wild emmer-derived powdery mildew resistance gene distal of breakpoint 0.89 in deletion line 2BL-6. Based on disease response patterns, genomic origin and chromosomal location the resistance gene in Zecoi-1 is temporarily designated MlZec1.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available