Journal
ANALYST
Volume 130, Issue 4, Pages 528-533Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b414189j
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Funding
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R01EB000250] Funding Source: NIH RePORTER
- NIBIB NIH HHS [R01 EB000250] Funding Source: Medline
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Molecular Probes' Alexa Fluor dyes are generally used for biological labeling because of their ideal fluorescent properties, but here we detail Alexa Fluor 488's nanomolar sensitivity to free iron. Furthermore, the dye has been encapsulated into a polymer nanosphere by a microemulsion method, producing < 100 nm particles. These nanosensors, PEBBLEs (Probe Encapsulated By Biologically Localized Embedding) have micromolar sensitivity and are non-responsive to other metal ions of biological interest.
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