Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 60, Issue 1, Pages 55-62Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2004.08.011
Keywords
microplate assay; bacterial adhesion; molecular mimicry; antiadhesive carbohydrates
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Adhesion of bacteria to the mucosal epithelial cell surface is the first step in infection, and studies have shown that inhibition of this step may be useful therapeutically. To test compounds that may prevent bacterial binding to a number of epithelial cell lines, we have developed a high-throughput adhesion assay using a microtitre plate system and bacteria that have been modified to express firefly luciferase. This method has proved to be a sensitive, rapid, and reproducible system for screening antiadhesive agents for their effects on bacterial adhesion. 2004 Elsevier B.V. All rights reserved.
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