Journal
FOOD RESEARCH INTERNATIONAL
Volume 38, Issue 1, Pages 45-50Publisher
ELSEVIER
DOI: 10.1016/j.foodres.2004.07.005
Keywords
antioxidative activity; Alaska pollack frame protein; hydrolysate; electron spin resonance
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Alaska pollack frame protein, which is normally discarded as an industrial by-product in the process of fish plant, was hydrolyzed with mackerel intestine crude enzyme (MICE). Alaska pollack frame protein hydrolysate (APH) was fractionated according to the molecular basis into five major types of APH-I (30-10 kDa), APH-II (10-5 kDa). APH-III (5-3 kDa), APH-IV (3-1 kDa), and APH-V (below 1 kDa) using an ultrafiltration (UF) membrane bioreactor system. The antioxidative activity of the APHs was investigated and compared with that of a natural antioxidant, alpha-tocopherol, used as a reference. The fraction, APH-V, exhibited the highest antioxidative activity was further purified using consecutive chromatographic methods on SP-Sephadex C-25 column, Sephadex G-25 column, and high-performance liquid chromatography (HPLC) on an octadecylsilane column. The sequence of the purified peptide was Leu-Pro-His-Ser-Gly-Tyr and molecular weight was 672 Da. In addition, the purified peptide scavenged 35% on hydroxyl radical at 53.6 muM using electron spin resonance (ESR) spectroscopy. (C) 2004 Elsevier Ltd. All rights reserved.
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