Journal
NUCLEAR MEDICINE AND BIOLOGY
Volume 32, Issue 1, Pages 29-38Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.nucmedbio.2004.09.004
Keywords
copper-64; azamacrocycle; HPLC; radio-LC-MS; impurity identification; metabolite analysis
Funding
- NCI NIH HHS [CA64475, CA93375, R24 CA86307] Funding Source: Medline
- NIGMS NIH HHS [GM08785-01, GM55916-01] Funding Source: Medline
- NATIONAL CANCER INSTITUTE [R29CA064475, R01CA064475, R01CA093375, R24CA086307] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R15GM055916, T32GM008785] Funding Source: NIH RePORTER
Ask authors/readers for more resources
The cross-bridged tetraamine ligand 4,11-bis(carboxymethyl)-1,4,8,11-tetraazabicyclo[6.6.2]hexadecane (H2CB-TE2A) allows formation of a radio-copper complex with higher in vivo stability than that of the corresponding non-cross-bridged analog 1,4,8,11-tetraazacyclotetradecane-1,4,8,11-tetraacetic acid (TETA). The structure of the Cu-nat(II) complex of CB-TE2A has been previously determined by X-ray crystallography; however, direct high-pressure liquid chromatography (HPLC) characterization of the corresponding Cu-64 complex was inaccessible due to the inability to detect the complex by ultraviolet absorbance at the radiotracer level. A reverse-phase HPLC separation of a series of Cu-nat(II)-tetraazamacrocyclic complexes, both traditional and cross-bridged, was developed and applied toward characterization and assessment of the purity of the corresponding no-carrier-added Cu-64-labeled complexes. Verification of the identity of copper-64-labeled compounds was also achieved by coupling this HPLC method with mass spectrometry. The radio-liquid chromatography/mass spectrometry methodology was further extended to study the in vivo metabolic fates of Cu-64-azarnacrocyclic complexes. (C) 2005 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available