Journal
GREEN CHEMISTRY
Volume 7, Issue 9, Pages 655-658Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b504689k
Keywords
-
Ask authors/readers for more resources
A novel type of ionic liquid (IL) sol-gel hybrid material was developed for amperometric biosensor application. This material was prepared by the hydrolysis of tetraethyl orthosilicate in a 1-butyl-3-methylimidazolium tetrafluoroborate (BMIM+BF4-) solution. The viscous IL prevented the cracking of the sol-gel derived glasses. Horseradish peroxidase (HRP) was immobilized in the hybrid material for the production of an amperometric hydrogen peroxide biosensor. The IL sol-gel enzyme electrodes retained the high activity of HRP and provided long-term stability of HRP in storage. The morphology of the matrix-containing enzyme was characterized by scanning electron microscopy. The characteristics of the biosensor were also investigated by cyclic voltammetry and chronoamperometry. In the presence of ferrocene as a mediator, the biosensor exhibited an excellent stability and sensitivity, and the linear calibration ranged from 0.02 to 0.26 mM with a detection of 1.1 mu M. The apparent Michaelis-Menten constant of the immobilized HRP was 2.0 mM.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available