Journal
GENETICS AND MOLECULAR BIOLOGY
Volume 28, Issue 3, Pages 548-554Publisher
SOC BRASIL GENETICA
DOI: 10.1590/S1415-47572005000400009
Keywords
Eucalyptus; protoporphyrinogen IX oxidase; catalase; peroxidase; herbicide
Funding
- RIPASA S. A. Celulose e Papel
- DURATEX
- Votorantim Celulose e Papel
- Suzano/Bahia Sul
- FAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo)
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This work was aimed at locating Eucalyptus ESTs corresponding to the PROTOX or PPO enzyme (Protoporphyrinogen IX oxidase, E. C. 1.3.3.4) directly related to resistance to herbicides that promote oxidative stress, changing the functionality of this enzyme. PROTOX, which is the site of action of diphenyl-ether (oxyfluorfen, lactofen, fomesafen), oxadiazole (oxadiazon and oxadiargyl), and aryl triazolinone (sulfentrazone and carfentrazone) herbicides, acts on the synthesis route of porphyrins which is associated with the production of chlorophyll a, catalases, and peroxidases. One cluster and one single read were located, with e-values better than e-70, associated to PROTOX. The alignment results between amino acid sequences indicated that this enzyme is adequately represented in the ESTs database of the FORESTs project.
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