Journal
CARCINOGENESIS
Volume 32, Issue 9, Pages 1333-1339Publisher
OXFORD UNIV PRESS
DOI: 10.1093/carcin/bgr128
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Funding
- Ministry of Education, Culture, Sports, Science, and Technology of Japan [20599008, 22-2434, 21229009, 23590937]
- Research Foundation of Translational Research Center
- Foundation Kyoto Cancer Society
- Fujiwara Memorial Foundation
- Grants-in-Aid for Scientific Research [20599008, 22790642, 23590937, 21229009, 09J40033, 10J02434, 23590361] Funding Source: KAKEN
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Macrophages are a major component of tumor stroma. Tumor-associated macrophages (TAMs) show anti- (M1) or protumor (M2) functions depending on the cytokine milieu of the tumor microenvironment. Cyclooxygenase-2 (COX-2) is constitutively expressed in a variety of tumors including colorectal cancer. TAMs are known to be a major source of COX-2 in human and mice intestinal tumors. COX-2 inhibitor reduces the number and size of intestinal adenomas in familial adenomatous polyposis patients and Apc(Min/+) mice. Although COX-2 inhibitor is thought to regulate cancer-related inflammation, its effect on TAM phenotype remains unknown. Here, we examined the effects of COX-2 inhibition on TAM phenotype and cytokine expression both in vivo and in vitro. Firstly, the selective COX-2 inhibitor celecoxib changed the TAM phenotype from M2 to M1, in proportion to the reduction in number of Apc(Min/+) mouse polyps. Concomitantly, the expression of M1-related cytokine interfron (IFN)-gamma was significantly upregulated by celecoxib, although the M2-related cytokines interleukin (IL)-4, IL-13 and IL-10 were not significantly altered. Secondly, IFN-gamma treatment attenuated M2 phenotype of mouse peritoneal macrophages and oriented them to M1 even in the presence of M2-polarizing cytokines such as IL-4, IL-13 and IL-10. Thus, our results suggest that COX-2 inhibition alters TAM phenotype in an IFN-gamma-dependent manner and subsequently may reduce intestinal tumor progression.
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