Journal
CARBON
Volume 49, Issue 10, Pages 3284-3291Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.carbon.2011.04.002
Keywords
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Funding
- Japan Society for the Promotion of Science [22003575]
- Ministry of Education, Science, Culture and Sport of Japan [22390361]
- Grants-in-Aid for Scientific Research [10J03575, 21659449, 22390361] Funding Source: KAKEN
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Surface coating treatment with multiwalled carbon nanotubes (MWCNTs) was applied to 3D collagen scaffold for bone tissue engineering. Rat primary osteoblasts (ROBs) were cultured on an MWCNT-coated collagen sponge (MWCNT-coated sponge) in a 3D dynamic flow cell culture system and differentiation markers were measured. Alkaline phosphatase activity at 1 day, and calcium and osteopontin contents of the MWCNT-coated sponges at 7 days were significantly higher than those of uncoated sponges. ROBs on the MWCNT-coated sponge differentiated earlier than on the uncoated sponge. In addition, the tissue response to the MWCNT-coated sponge was evaluated. Slight inflammation was observed around MWCNTs at 7 and 28 days after implantation in subcutaneous tissue. Significantly more bone formation was observed around the MWCNT-coated sponges than around the uncoated sponges and new bone attached to MWCNTs directly at 28 and 56 days after implantation in the femur. Moreover, at 28 days after implantation of the MWCNT-coated sponge with osteoblasts cultured for 1 day, bone tissues were successfully formed in the pores according to its honeycomb structure. Therefore, MWCNT coating appears to be effective for bone tissue engineering. (C) 2011 Elsevier Ltd. All rights reserved.
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