Journal
BRAZILIAN JOURNAL OF MICROBIOLOGY
Volume 36, Issue 1, Pages 63-69Publisher
SPRINGER
DOI: 10.1590/S1517-83822005000100013
Keywords
thermophilic fungi; Aspergillus fumigatus; pectinase; polygalacturonase
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A thermophilic fungal strain producing both pectinase and polygalacturonase was isolated after primary screening of 120 different isolates. The fungus was identified as Aspergillus fumigatus Fres. MTCC 4163. Using solid-state cultivation, the optimum levels of variables for pectinase and polygalacturonase (PG) production were determined. Maximal levels of enzyme activities were achieved upon growing the culture in a medium containing wheat bran, sucrose, yeast extract and (NH4)(2)SO4 after 2-3 days of incubation at a temperature of 50 degrees C. Highest enzyme activities of 1116 Ug(-1) for pectinase and 1270 Ug(-1) for polygalacturonase were obtained at pH 4.0 and 5.0, respectively.
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