Journal
WATER QUALITY RESEARCH JOURNAL OF CANADA
Volume 40, Issue 2, Pages 145-154Publisher
IWA PUBLISHING
DOI: 10.2166/wqrj.2005.017
Keywords
peroxidase; endocrine disruptors; xenoestrogens; alkylphenols
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The xenoestrogen alkylphenols 4-nonylphenol (3.4 mg/L) and octylphenol (6.0 mg/L) were oxidized by hydrogen peroxide using horseradish peroxidase (HRP) as a biocatalyst. Substrate transformation required about one mole of peroxide per mole of phenolic compound. A high degree of conversion of alkylphenol was achieved within a 3-h reaction time. In the case of 4-nonylphenol, HRP treatment led to complete disappearance of Microtox toxicity. Results of the yeast estrogen screen (YES) assay demonstrated that the reaction products of HRP-catalyzed 4-nonylphenol conversion lacked estrogenic activity. A new approach to the YES assay has been suggested based on observations made during this study.
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