4.7 Article

Oral immunotherapy against a pollen allergy using a seed-based peptide vaccine

Journal

PLANT BIOTECHNOLOGY JOURNAL
Volume 3, Issue 5, Pages 521-533

Publisher

WILEY
DOI: 10.1111/j.1467-7652.2005.00143.x

Keywords

edible vaccine; endosperm expression; immune tolerance; Japanese cedar pollen; pollinosis; T-cell epitope; transgenic rice

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Peptide immunotherapy using dominant T-cell epitopes is safer and more effective than conventional immunotherapy for the treatment of immunoglobulin E (IgE)-mediated allergic diseases. When allergenic T-cell epitope peptides are expressed in the edible part of transgenic plants, successful mucosal immune tolerance to these allergens may be attainable by the consumption of these plants. In this study, we generated transgenic rice seed that accumulated high concentrations (about 60 mu g per grain) of polypeptide consisting of seven dominant human T-cell epitopes derived from the Japanese cedar pollen allergens, Cry j 1 and Cry j 2, in the endosperm. Oral administration of these transgenic rice seeds to B10.S mice before or after they were immunized with Cry j 1 holoprotein reduced not only their T-cell proliferative response to Cry j 1, but also their serum IgE levels, proving the efficacy of oral immunotherapy for the treatment of pollinosis.

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