Journal
CARBOHYDRATE RESEARCH
Volume 385, Issue -, Pages 58-64Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2013.12.009
Keywords
ESI-CID-MS/MS; Laminaran; Sargassum fusiforme; NMR; Sulfated polysaccharide
Funding
- National Key Technology Research and Development Program [2013BAB01B01]
- Ocean Public Welfare Scientific Research Project [201005024, 201405040]
- Jiangsu Science and Technology Project [BE2012687]
- Special Fund for Cooperation between Jilin Province
- Chinese Academy of Sciences [2013SYHZ0023]
- Applied Research Projects of the Nantong Municipality [BK2013011]
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The crude polysaccharide (HFS) from Sargassum fusiforme (Hizikia fusiforme) was extracted using 0.1 M HCl and was fractionated by anion-exchange chromatography into three fractions: HFS-1, HFS-2, and HFS-3. Based on the chemical analysis, HFS-1 was composed of laminaran, HFS-2 was a mixture of alginate and sulfated heteropolysaccharides, and HFS-3 was primarily composed of sulfated galactofucan. The NMR spectra revealed that HFS-1 was composed of a soluble laminaran with chains that are terminated by beta-D-glucose residues. In contrast, the spectra obtained for HFS-2 were still complex, even after most of the alginate was removed. In addition, HFS-3 might contain 3-linked fucan sulfated at C-2, 6-linked galactan sulfated at C-2 and branched at C-4 by 2-sulfated Fuc, and galactofucan with a backbone of either alternating Gal and Fuc sulfated at C-2 or alternating (Gal)(n) and (Fuc)(n) sulfated at C-2. Moreover, HFS-3 also contained small amounts of fucoglucuronomannan and xylan. (C) 2013 Elsevier Ltd. All rights reserved.
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