4.5 Article

Assessing acceptor substrate promiscuity of YjiC-mediated glycosylation toward flavonoids

Journal

CARBOHYDRATE RESEARCH
Volume 393, Issue -, Pages 26-31

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2014.03.011

Keywords

Flavonoids; Glycosyltransferase; Natural product glucosides; Non-regiospecific glycosylation

Funding

  1. Intelligent Synthetic Biology Center of Global Frontier Project - Ministry of Education, Science and Technology [2011-0031960]
  2. Next-Generation BioGreen 21 Program [JKS: PJ0094832]
  3. Rural Development Administration
  4. Republic of Korea

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The acceptor substrate promiscuity of YjiC, a UDP-glycosyltransferase from Bacillus licheniformis, was explored with seven different classes (flavonols, flavanols, flavones, flavanones, chalcone, stilbene, and isoflavonoids) of 23 flavonoid acceptors. For most of the polyphenols used in the reactions, the enzymatic bioconversion was significantly higher with the production of multiple glucosylated derivatives. This study highlights the highly flexible non-regiospecific glycosylation ability of YjiC toward polyphenolic compounds. The catalytic potential of YjiC could be useful to generate a library of natural product glucosides. (C) 2014 Elsevier Ltd. All rights reserved.

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