Lipopolysaccharide structure of Helicobacter pylori serogroup O:3

In this study, we describe a re-investigation of the lipopolysaccharide structure of Helicobacter pylori serogroup O:3. Application of NMR and MS approaches to the analysis of oligosaccharides obtained through degradation of LPS from H. pylori serogroup O:3 by various methods confirmed that its general architecture was identical to that of LPS from H. pylori strains 26695 and SS1 and followed a sequential linear assembly of the alpha-1,6-glucan, DD-heptan, and O-chain components. Additionally, MALDI-MS analysis demonstrated that a significant proportion of H. pylori serogroup O:3 LPS was terminated with alpha-1,6-glucan and was not further substituted by DD-heptan and the O-chain polysaccharide. Crown Copyright (C) 2013 Published by Elsevier Ltd. All rights reserved.