Journal
CARBOHYDRATE RESEARCH
Volume 381, Issue -, Pages 33-42Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2013.08.024
Keywords
Xylan; Hemicellulose; Acetyl; Feruloyl; Enzyme resistant; Ethyl glycoside
Funding
- Dutch Ministry of Economic Affairs via an EOS-KTO grant
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In order to use corn fiber as a source for bioethanol production the enzymatic hydrolysis of the complex glucuronoarabinoxylans present has to be improved. Several oligosaccharides present in the supernatant of mild acid pretreated and enzymatically saccharified corn fiber that resist the current available enzymes were (semi) purified for structural analysis by NMR or ESI-MSn. The structural features of 21 recalcitrant oligosaccharides are presented. A common feature of almost all these oligosaccharides is that they contain (part of) an alpha-L-galactopyranosyl-(1 -> 2)-beta-D-xylopyranosyl-(1 -> 2)-5-O-trans-feruloyl-L-arabinofuranose side chain attached to the O-3 position of the beta-1-4 linked xylose backbone. Several of the identified oligosaccharides contained an ethyl group at the reducing end hypothesized to be formed during SSF. The ethyl glycosides found are far more complex than previously described structures. A new feature present in more than half of the oligosaccharides is an acetyl group attached to the O-2 position of the same xylose to which the oligomeric side chain was attached to the O-3 position. Finding enzymes attacking these large side chains and the dense substituted xylan backbone will boost the hydrolysis of corn fiber glucuronoxylan. (c) 2013 Elsevier Ltd. All rights reserved.
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