Journal
CARBOHYDRATE RESEARCH
Volume 352, Issue -, Pages 60-64Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2012.02.009
Keywords
Silver nanoparticles; Starch; Amylase; Nanocatalysis; Degradation; Immobilized enzymes
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Silver nanoparticles (AgNPs) are proven to be an effective catalytic material for various applications due to their excellent optical and electronic properties. In this paper, we describe a novel approach for the degradation of starch using the catalytic behaviour of AgNPs in an enzyme catalysed reaction of starch hydrolysis by alpha-amylase. AgNPs were synthesized by soluble starch reducing silver nitrate to silver atoms. An increase of 4.7-fold in reducing sugar formation and 1.5 times faster enzyme activity confirmed the catalytic activity of AgNPs as a nanocatalyst. Surprisingly, starch degradation tests revealed that 9.9 mg of starch was hydrolysed within 5 min, which corroborates with the reducing sugar assay. In short, the present study paves way for the faster degradation of starch by immobilizing the enzyme onto the surface of the AgNP, which could be a promising application in the food industry. (C) 2012 Elsevier Ltd. All rights reserved.
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