Discovery of structurally diverse natural product antagonists of chemokine receptor CXCR3

The chemokines (CXCL9, CXCL10 and CXCL11) and associated CXCR3 receptor are expressed during the inflammatory process from multiple sclerosis, atherosclerosis or organ transplantation resulting in the recruitment of lymphocytes leading to tissue damage. It is hypothesized that blocking of the ligand/CXCR3 receptor interaction has potential to provide opportunity for development of agents that would block tissue rejection. In this paper, four classes of natural product inhibitors (IC(50) ranging 0.1-41 mu M) have been described that block the CXCR3 receptor interaction of IP-10 ligand. These include a cyclic thiopeptide (duramycin), polyketide glycosides (roselipins), steroidal glycosides (hypoglausin A and dioscin) and a novel alkyl pyridinium alkaloid that were isolated by bioassay-guided fractionation of the organic extracts derived from actinomycete, fungal, plant and marine sources and discovered using (125)I IP-10/CXCR3 binding assay. Duramycin was the most potent with an IC(50) of 0.1 mu M. Roselipins 2A, 2B and 1A showed IC(50) values of 14.6, 23.5, and 41 mu M, respectively. Diosgenin glycosides dioscin, hypoglaucin A and kallstroemin D exhibited IC(50) values of 2.1, 0.47 and 3 mu M, respectively. A novel cyclic 3-alkyl pyridinium salt isolated from a sponge displayed a binding IC(50) of 0.67 mu M.