Journal
CARBOHYDRATE RESEARCH
Volume 346, Issue 7, Pages 973-977Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2011.03.024
Keywords
Acinetobacter baumannii; Capsular polysaccharide; Western blot; Structural analysis
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The structures of the capsular polysaccharides (CPSs) of the two clinical isolates Acinetobacter baumannii SMAL and MG1 were elucidated. Hot phenol/water extractions of the dry biomasses, followed by enzymatic digestions and repeated ultracentrifugations led to the isolation of polysaccharides that were negative in Western blot analysis utilizing an anti-lipid A antibody, thus proving that they were not the LPS O-antigens but CPSs. Their structures were established on the basis of NMR spectroscopy and GC-MS analyses. The A. baumannii MG1 CPS consisted of a linear aminopolysaccharide with acyl substitution heterogeneity at the N-4 amino group of QuipN4N: 4)-alpha-D-GlcpNAc-(1 -> 4)-alpha-L-GalpNAcA-(1 -> 3)-beta-D-QuipNAc4NR-(1 -> R = -3-hydroxybutyrryl or acetyl. The repeating unit of the CPS produced by strain SMAL is a pentasaccharide, already reported for the O-antigen moiety from A. baumannii strain ATCC 17961: beta-D-GlcpNAc3NAcA-(1 down arrow 4) 6)-beta-D-Glcp-(1 -> 3)-beta-D-GalpNAc-(1 -> 3)-alpha-D-Galp-(1 -> 6)up arrow beta-D-GlcpNAc-(1 (C) 2011 Elsevier Ltd. All rights reserved.
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