Journal
CARBOHYDRATE RESEARCH
Volume 345, Issue 1, Pages 108-114Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2009.09.015
Keywords
Lactobacillus johnsonii; Exopolysaccharide; Lactic acid bacteria; Crohn disease; Inflammatory bowel disease; Probiotic
Funding
- Ministry of Sciences and Higher Education [N401 332036]
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This paper describes the structure of neutral exopolysaccharide (EPS) produced by Lactobacillus johnsonii 142, strain of the lactic acid bacteria isolated from the intestine of mice with experimentally induced inflammatory bowel disease (IBD). Sugar and methylation analyses along with H-1 and C-13 NMR spectroscopy, including two-dimensional H-1,H-1 COSY,TOCSY, NOESY, and H-1, C-13 HSQC experiments revealed that the repeating unit of the EPS is a pentasaccharide: -> 3)-alpha-D-Galp-(1 -> 3)-beta-D-Glcp-(1 -> 5)-beta-D-Galf-(1 -> 3)-alpha-D-Galp-(1 -> 3)-alpha-D-Galp-(1 -> The rabbit antiserum raised against whole cells of L johnsonii 142 reacted with homologous EPS, and cross-reacted with exopolysaccharide from Lactobacillus animalis/murinus 148 isolated also from mice with IBD, but not reacted with EPS of L johnsonii 151 from healthy mice. (C) 2009 Elsevier Ltd. All rights reserved.
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