Journal
CARBOHYDRATE RESEARCH
Volume 345, Issue 10, Pages 1389-1393Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carres.2010.02.017
Keywords
Chondrocytes; Cytokines; Glycosylation; Glycosyltransferases; RT-qPCR; LC-ESI-MS
Ask authors/readers for more resources
Despite the significance of glycoproteins for extracellular matrix assembly in cartilage tissue, little is known about the regulation of the chondrocyte glycophenotype under inflammatory conditions. The present study aimed to assess the effect of IL-1 beta and TNF-alpha on specific features of the glycophenotype of primary human chondrocytes in vitro. Using LC-MS, we found that both cytokines increased overall sialylation of N- and O-glycans and induced a shift towards alpha-(2 -> 3)-linked sialic acid residues in chondrocyte glycoproteins. These results were supported by quantitative PCR showing increased expression of alpha-(2 -> 3) sialyltransferases in treated cells. Moreover, we found that both IL-1 beta and TNF-alpha induced a considerable shift from oligomannosidic glycans towards complex-type N-glycans. In contrast, core alpha-(1 -> 6)-fucosylation of chondrocyte N-glycans was found to be reduced particularly by TNF-alpha. In summary, inflammatory conditions induce specific alterations of the chondrocyte glycophenotype which might affect cell-matrix interactions or the function of endogenous lectins. (C) 2010 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available